Molecular Cloning: A Laboratory Manual has always been the one indispensable molecular biology laboratory manual for protocols and techniques. The 4th edition of this classic manual preserves the detail and clarity of previous editions as well as the theoretical and historical underpinnings of the techniques presented. Ten original core chapters reflect developments and innovation in standard techniques and introduce new cutting-edge protocols. Twelve entirely new chapters are devoted to the most exciting current research strategies, including epigenetic analysis, RNA interference, genome sequencing, and bioinformatics. This manual is essential for both the inexperienced and the advanced user.